Improved serum lipoprotein electrophoresis procedure in polyacrylamide gradient gel.

A method based based upon polyacrylamide gradient gel for the electrophoretic separation of lipoproteins prestained with the diformazan of nitroblue tetrazolium is described and evaluated. It gives accurate characterization of the different lipoproteins, good separation between chylomicrons and very low density lipoproteins and can be also applied to the diagnosis of type III hyperlipoproteinemia without need for ultracentrifugation.