Ando Miyuki, Tomoyori Hiroko, Imaizumi Katsumi
Laboratory of Nutrition Chemistry, Division of Bioresource and Bioenvironmental Sciences, Graduate School, Kyushu University, Fukuoka 812-8581, Japan.
Br J Nutr. 2002 Oct;88(4):339-45. doi: 10.1079/BJN2002670.
There are conflicting reports regarding the effect of dietary cholesterol-oxidation products (oxysterols) on the development of atherosclerosis in experimental animals. To address this issue, apolipoprotein (Apo) E-deficient mice were fed a purified diet (AIN-93) or the same purified diet containing 0.2 g cholesterol or 0.2 g oxysterols/kg. The dietary oxysterols had no significant effect on the serum lipid levels. Although all of the diet-derived oxysterols (cholest-5-en-3beta,7alpha-diol, cholest-5-en-3beta,7beta-diol, cholestan-5alpha,6alpha-epoxy-3beta-ol, cholestan-5beta,6beta-epoxy-3beta-ol, cholestan-3beta, 5alpha, 6beta-triol, cholest-5-en-3beta-ol-7-one and cholest-5-en-3beta, 25-diol) accumulated in the serum and liver, only cholest-5-en-3beta-ol-7-one and cholestan-3beta, 5alpha, 6beta-triol accumulated significantly (P<0.05) in the aorta. The oxysterol diet did not result in elevation of the aortic cholesterol level or the lesion volume in the aortic valve. These present results indicate that exogenous oxysterols do not promote the development of atherosclerosis in ApoE-deficient mice.
关于膳食胆固醇氧化产物（氧化甾醇）对实验动物动脉粥样硬化发展的影响，存在相互矛盾的报道。为解决这一问题，给载脂蛋白（Apo）E缺乏的小鼠喂食纯化饮食（AIN-93）或含有0.2 g胆固醇或0.2 g氧化甾醇/千克的相同纯化饮食。膳食氧化甾醇对血清脂质水平无显著影响。尽管所有饮食来源的氧化甾醇（胆甾-5-烯-3β,7α-二醇、胆甾-5-烯-3β,7β-二醇、胆甾烷-5α,6α-环氧-3β-醇、胆甾烷-5β,6β-环氧-3β-醇、胆甾烷-3β,5α,6β-三醇、胆甾-5-烯-3β-醇-7-酮和胆甾-5-烯-3β,25-二醇）都在血清和肝脏中蓄积，但只有胆甾-5-烯-3β-醇-7-酮和胆甾烷-3β,5α,6β-三醇在主动脉中显著蓄积（P<0.05）。氧化甾醇饮食并未导致主动脉胆固醇水平升高或主动脉瓣病变体积增加。这些目前的结果表明，外源性氧化甾醇不会促进ApoE缺乏小鼠动脉粥样硬化的发展。
