Ma Ningning, Koelling Kurt W, Chalmers Jeffrey J
Department of Chemical Engineering, The Ohio State University, 140W 19th Ave., Columbus, Ohio 43210, USA.
Biotechnol Bioeng. 2002 Nov 20;80(4):428-37. doi: 10.1002/bit.10387.
A microfluidic device was fabricated via photolithographic techniques which can create transient elongational and shear forces ranging over three orders of magnitude while still maintaining laminar flow conditions. The contractional fluid flow inside the microfluidic device was simulated with FLUENT (a computational fluid dynamics computer program) and the local deformation forces were characterized with the scalar quantity, local energy dissipation rate. The sensitivities of four cell lines (CHO, HB-24, Sf-9, and MCF7) were tested in the device. The results indicate that all four cell lines are able to withstand relatively intense energy dissipation rates (up to 10(4)-10(5) kW/m(3)), which is orders of magnitude higher than the maximum local energy dissipation rates generated by impellers in bioreactors, but comparable to that associated with small bursting bubbles. While the concept that suspended animal cells are relatively robust with respect to purely hydrodynamic forces in bioprocess equipment is well known, these results quantitatively demonstrate these observations.
通过光刻技术制造了一种微流体装置，该装置可以产生范围跨越三个数量级的瞬态拉伸力和剪切力，同时仍保持层流条件。使用FLUENT（一种计算流体动力学计算机程序）对微流体装置内的收缩流体流动进行了模拟，并用标量局部能量耗散率来表征局部变形力。在该装置中测试了四种细胞系（CHO、HB - 24、Sf - 9和MCF7）的敏感性。结果表明，所有四种细胞系都能够承受相对较高的能量耗散率（高达10⁴ - 10⁵kW/m³），这比生物反应器中叶轮产生的最大局部能量耗散率高出几个数量级，但与小破裂气泡相关的能量耗散率相当。虽然悬浮动物细胞在生物处理设备中对纯流体动力相对稳健这一概念是众所周知的，但这些结果定量地证明了这些观察结果。
