Karlén B, Garle M, Rane A, Gutova M, Lindborg B
Eur J Clin Pharmacol. 1975 Jun 13;8(5):359-63. doi: 10.1007/BF00562663.
A modified gas chromatographic procedure for the determination of unconjugated and conjugated 4-hydroxydiphenylhydantoin (4-OH-DPH) in urine has been developed. Unconjugated 4-OH-DPH is determined after selective extraction with toluene-ether (1:1). For the assay of conjugated 4-OH-DPH, the urine is pre-extracted with isoamylalcohol before acid hydrolysis to avoid interference by the dihydrodiol metabolite of DPH. The sensitivity of the method is 0.1 mug per ml. The method has been used to determine the urinary metabolites in two adult volunteers, during steady state plasma concentrations of DPH and in the elimination phase.
已开发出一种改良的气相色谱法，用于测定尿液中未结合和结合的4-羟基二苯乙内酰脲（4-OH-DPH）。用甲苯 - 乙醚（1:1）选择性萃取后测定未结合的4-OH-DPH。对于结合的4-OH-DPH的测定，尿液在酸水解前先用异戊醇预萃取，以避免DPH的二氢二醇代谢物的干扰。该方法的灵敏度为每毫升0.1微克。该方法已用于测定两名成年志愿者在DPH稳态血药浓度期间和消除阶段的尿液代谢物。
