Savchenko V K, Rokitskiĭ P F
Genetika. 1975;11(3):158-66.
The mitotic activity and ploidy of cells of three strains of cellus tissue by the addition of the culture medium of 0,01; 0,1 and 1 mg/l of phytohemagglutinin was studied. These strains derived from the leaves of haploid, diploid, and tetraploid plants of Lycopersicon, esculentum. The number of dividing cells under the influence of phytohemagglutinin increased by 1;5 and 4% for haploid, diploid and tetraploid strains respectively. The shift of the peak of the mitotic activity at early stages of subculture under the effect of phytohemagglutinin was noticed. Phytohemagglutinin did not change the frequencies of cells of different ploidy in all the strains analysed. A weak mutagenic activity of phytohemagglutinin in the concentrations used was observed. Cells with single bridges were more frequently observed among the aberrant cells.
研究了添加0.01、0.1和1mg/l植物血凝素培养基时,三种番茄组织细胞系的有丝分裂活性和倍性。这些细胞系分别来源于番茄单倍体、二倍体和四倍体植株的叶片。在植物血凝素的影响下,单倍体、二倍体和四倍体细胞系的分裂细胞数量分别增加了1%、5%和4%。注意到在植物血凝素作用下,继代培养早期有丝分裂活性峰值的偏移。植物血凝素在所有分析的细胞系中均未改变不同倍性细胞的频率。观察到在所使用浓度下植物血凝素具有微弱的诱变活性。在异常细胞中更频繁地观察到具有单桥的细胞。