Kloucek F
Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1975;193(4):269-85. doi: 10.1007/BF00417576.
In 11 patients, the clinical, histological and histochemical picture of an unusual corneal affection termed "corneal superficial unwettable defect", is reported. It develops during the healing of various corneal lesions. Clinically, it is described as a defect of the epithelium with a denuded and changed Bowman's membrane, the surface of which repells tears and cannot be stained with fluorescein. In the histological picture, the changed Bowman's membrane has fibrillar or foamy appearence. It stains strongly with colloidal iron and alcian blue, but PAS and toluidine blue reactions are weak. Histochemical reactions for neutral fats are strongly positive, but those for phospholipids are negative. The results of extractions speak for the presence of some lipid component, which is neither neutral fat nor phospholipid. Hence it is supposed that there may be some waxes-ointment bases, reacting with the pathologically changed Bowman's membrane. The possible mechanisms injuring or influencing the Bowman's membrane are also discussed.
本文报告了11例患有一种名为“角膜浅层不可湿润缺损”的罕见角膜病变患者的临床、组织学和组织化学表现。该病变在各种角膜损伤愈合过程中出现。临床上，它被描述为上皮缺损，伴有Bowman膜剥脱和改变，其表面排斥泪水且不能被荧光素染色。在组织学图像中，改变后的Bowman膜呈现出纤维状或泡沫状外观。它对胶体铁和阿尔辛蓝染色强烈，但PAS和甲苯胺蓝反应较弱。中性脂肪的组织化学反应呈强阳性，但磷脂的反应为阴性。提取结果表明存在某种脂质成分，它既不是中性脂肪也不是磷脂。因此推测可能存在一些蜡质 - 软膏基质，与发生病理改变的Bowman膜发生反应。文中还讨论了损伤或影响Bowman膜的可能机制。
