Ando Izuru, Tsukumo Yoshinori, Wakabayashi Tetsuya, Akashi Sachiko, Miyake Kensuke, Kataoka Takao, Nagai Kazuo
Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan.
Int Immunopharmacol. 2002 Jul;2(8):1155-62. doi: 10.1016/s1567-5769(02)00076-0.
Two active polysaccharide fractions (SF1 and SF2) purified from dried safflower petals (Carthamus tinctorius L.) stimulated the synthesis of various cytokines by peritoneal macrophages. In a number of cell types, SF1 and SF2 induced a rapid degradation of IkappaB alpha essential for the activation of the transcription factor NF-kappaB. Toll-like receptor 4 (TLR4), but not TLR2, was expressed in all cell lines that responded to SF1 and SF2. Enforced expression of TLR4 and MD-2 rendered responsiveness to SF1 and SF2. Moreover, these safflower polysaccharides failed to induce the production of TNF-alpha and NO by peritoneal macrophages prepared from C3H/HeJ mice that have a point mutation in the Tlr4 gene. Thus, these observations clearly indicate that safflower polysaccharides activate the NF-kappaB signaling pathway via TLR4.
从干红花花瓣（红花）中纯化得到的两种活性多糖组分（SF1和SF2）可刺激腹膜巨噬细胞合成多种细胞因子。在许多细胞类型中，SF1和SF2诱导了对转录因子NF-κB激活至关重要的IκBα的快速降解。在所有对SF1和SF2有反应的细胞系中均表达了Toll样受体4（TLR4），而非TLR2。TLR4和MD-2的强制表达使细胞对SF1和SF2产生反应。此外，这些红花多糖无法诱导由Tlr4基因存在点突变的C3H/HeJ小鼠制备的腹膜巨噬细胞产生TNF-α和NO。因此，这些观察结果清楚地表明红花多糖通过TLR4激活NF-κB信号通路。
