Boesken W H
Contrib Nephrol. 1975;1:143-55.
The SDS polyacrylamide gelelectrophoresis (SDS-PAA) as used in this study has proven to be an excellent tool to differentiate urinary proteins qualitatively and quantitatively, since the proteins are differentiated exclusively according to their molecular radius. Selectivity was estimated by the ratio transferrin:IgG. Some of the proteins were identified by specific antisera. For clinical use SDS-PAA may distinguish: chronic glomerulonephritis from chronic pyelonephritis; the different diabetic nephropathies; some cases of minimal change nephritis from proliferative and degenerative glomerular diseases; the uncomplicated posttransplantation course from (interstitial) rejection crises and from glomerular diseases (recurrent GN, glomerular rejection disease), and the persisting small glomerular proteinuria after acute glomerulonephritis from proteinurias becoming physiological.
本研究中使用的十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAA）已被证明是定性和定量区分尿蛋白的优秀工具，因为蛋白质仅根据其分子半径进行区分。通过转铁蛋白与免疫球蛋白G的比率来评估选择性。一些蛋白质通过特异性抗血清进行鉴定。对于临床应用，SDS-PAA可以区分：慢性肾小球肾炎与慢性肾盂肾炎；不同类型的糖尿病肾病；一些微小病变性肾炎病例与增殖性和退行性肾小球疾病；单纯的移植后病程与（间质性）排斥反应危机以及肾小球疾病（复发性肾小球肾炎、肾小球排斥病），以及急性肾小球肾炎后持续存在的少量肾小球蛋白尿与生理性蛋白尿。
