树突状细胞亚群上结合HIV的受体的多样性。

Diversity of receptors binding HIV on dendritic cell subsets.

作者信息

Turville Stuart G, Cameron Paul U, Handley Amanda, Lin George, Pöhlmann Stefan, Doms Robert W, Cunningham Anthony L

机构信息

Center for Virus Research, Westmead Millennium Institute, Westmead Hospital and University of Sydney, Sydney, NSW 2145, Australia.

出版信息

Nat Immunol. 2002 Oct;3(10):975-83. doi: 10.1038/ni841. Epub 2002 Sep 23.

DOI:10.1038/ni841

PMID:12352970

Abstract

The ability of HIV-1 to use dendritic cells (DCs) for transport and to transfer virus to activated T cells in the lymph node may be crucial in early HIV-1 pathogenesis. We have characterized primary DCs for the receptors involved in viral envelope attachment and observed that C-type lectin receptor (CLR) binding was predominant in skin DCs, whereas binding to emigrating and tonsil DCs was CD4-dependent. No one CLR was solely responsible for envelope binding on all skin DC subsets. DC-SIGN (DC-specific ICAM-3-grabbing nonintegrin) was only expressed by CD14(+)CDla(lo) dermal DCs. The mannose receptor was expressed by CD1a(hi) and CD14(+)CDla(lo) dermal DCs, and langerin was expressed by Langerhans cells. The diversity of CLRs able to bind HIV-1 in skin DCs may reflect their ability to bind a range of microbial glycoproteins.

摘要

人类免疫缺陷病毒1型（HIV-1）利用树突状细胞（DC）进行转运并将病毒传递至淋巴结中活化的T细胞的能力，在HIV-1早期发病机制中可能至关重要。我们已对参与病毒包膜附着的受体的原代DC进行了表征，并观察到C型凝集素受体（CLR）结合在皮肤DC中占主导地位，而与迁移和扁桃体DC的结合则依赖于CD4。没有一种CLR单独负责所有皮肤DC亚群上的包膜结合。DC-SIGN（DC特异性细胞间黏附分子3抓取非整合素）仅由CD14(+)CD1a(lo)真皮DC表达。甘露糖受体由CD1a(hi)和CD14(+)CD1a(lo)真皮DC表达，而朗格汉斯细胞表达朗格蛋白。能够在皮肤DC中结合HIV-1的CLR的多样性可能反映了它们结合一系列微生物糖蛋白的能力。