Chang L R
Chin J Physiol. 1975 Dec 31;22(1):39-46.
The effects of three natriuretic agents on Na transport across the mouse intestine were determined. It was found that ethacrynic acid at a concentration of 1mM, markedly reduced the net transmural transport of Na ion by decreasing themucosa-to-serosa flux with little or no effect on serosa-to-mucosa flux. Furosemide or amiloride at a concentration of 1 mM produced similar but less profound effects. A reduction of net Na22 flux was observed in the presence of either diuretic, but with furosemide and amiloride this inhibitory effect observed only if glucose was present in the bathing solution. It is, therefore, concluded that the intestinal transport of Na ion involves two processes, one is glucose or substrate-dependent, and the other glucose or substrate-independent. Ethacrynic acid inhibits the substrate-independent process while furosemide and amiloride affect only the substrate-dependent process of Na transport.
研究了三种利钠剂对小鼠肠道钠转运的影响。结果发现，浓度为1mM的依他尼酸通过降低黏膜到浆膜的通量，显著降低了钠离子的净跨壁转运，而对浆膜到黏膜的通量几乎没有影响。浓度为1mM的呋塞米或阿米洛利产生了类似但较弱的效果。在任何一种利尿剂存在的情况下，均观察到净Na22通量降低，但只有在浴液中存在葡萄糖时，呋塞米和阿米洛利才会出现这种抑制作用。因此，可以得出结论，钠离子的肠道转运涉及两个过程，一个是葡萄糖或底物依赖性的，另一个是葡萄糖或底物非依赖性的。依他尼酸抑制底物非依赖性过程，而呋塞米和阿米洛利仅影响钠离子转运的底物依赖性过程。
