Ritchey J W, Ealey K A, Payton M E, Eberle R
Department of Veterinary Pathobiology, College of Veterinary Medicine, Oklahoma State University, Stillwater, OK, 74078, USA.
J Comp Pathol. 2002 Aug-Oct;127(2-3):150-61. doi: 10.1053/jcpa.2002.0575.
The comparative pathology of Herpesvirus papio 2 (HVP2) of baboons and SA8 virus of African green monkeys relative to that of herpes simplex virus (HSV1) of man was investigated in young adult mice inoculated intramuscularly and observed for 21 days. The 50% infectious dose (ID(50)) for HVP2 was approximately 10(2.0) plaque-forming units (PFU), while the ID(50) for HSV1 and SA8 was 10(2.5) and 10(3.8), respectively. There were marked differences in the ability of these three viruses to invade the central nervous system (CNS) and cause clinical neurological disease. HSV1 produced neurological signs in a few animals given 10(6)PFU, but SA8 did not. In contrast, HVP2 readily invaded the CNS and produced fatal disease with doses as low as 10(2)PFU. Two isolates of HVP2 tested had a 50% CNS disease dose (CNSD(50)) of 10(2.5) and 10(3.0)PFU and an LD(50) of 10(3.8) and 10(4.3)PFU, respectively. Histopathological examination of tissue from HVP2-infected mice revealed severe lesions of inflammation and necrosis in the central, peripheral and autonomic nervous systems, as well as of other tissues including skin, adrenal glands and the gastrointestinal tract. Viral antigens were detected immunohistochemically in lesions. This study showed that while both HVP2 and SA8 could infect mice, there were marked differences in the ability of these two closely related viruses to cause clinical disease and CNS lesions. This murine model may prove useful in the investigation of viral or host determinants responsible for the varying neurovirulence of these simian alpha-herpesviruses.
在年轻成年小鼠中进行了肌肉注射，并观察21天，研究了狒狒的疱疹病毒2型（HVP2）和非洲绿猴的SA8病毒相对于人类单纯疱疹病毒（HSV1）的比较病理学。HVP2的50%感染剂量（ID(50)）约为10(2.0)个空斑形成单位（PFU），而HSV1和SA8的ID(50)分别为10(2.5)和10(3.8)。这三种病毒侵入中枢神经系统（CNS）并引起临床神经疾病的能力存在显著差异。给予10(6)PFU的少数动物中，HSV1产生了神经症状，但SA8没有。相比之下，HVP2很容易侵入CNS，低至10(2)PFU的剂量就能导致致命疾病。测试的两株HVP2的50%中枢神经系统疾病剂量（CNSD(50)）分别为10(2.5)和10(3.0)PFU，半数致死剂量（LD(50)）分别为10(3.8)和10(4.3)PFU。对HVP2感染小鼠的组织进行组织病理学检查发现，中枢、外周和自主神经系统以及包括皮肤、肾上腺和胃肠道在内的其他组织出现严重的炎症和坏死病变。在病变中通过免疫组织化学检测到病毒抗原。这项研究表明，虽然HVP2和SA8都能感染小鼠，但这两种密切相关的病毒引起临床疾病和CNS病变的能力存在显著差异。这种小鼠模型可能在研究导致这些猿猴α疱疹病毒不同神经毒力的病毒或宿主决定因素方面证明是有用的。
