Fukami Hiroyuki, Asakura Tomiko, Hirano Hiroshi, Abe Keiko, Shimomura Koichiro, Yamakawa Takashi
Laboratory of Plant Biotechnology, Department of Global Agricultural Sciences, The University of Tokyo, Japan.
Plant Cell Physiol. 2002 Sep;43(9):1054-8. doi: 10.1093/pcp/pcf119.
In Atropa belladonna hairy roots, exogeneously added salicylic acid (SA) is converted to methyl salicylate (MSA) through the reaction, which might be catalysed by S-adenosyl-L-methionine: salicylic acid carboxyl methyltransferase (SAMT). Here we cloned a cDNA for A. belladonna SAMT (AbSAMT1), which consisted of 357 aa residues. It was expressed in E. coli, and the recombinant AbSAMT1 showed SAMT activity. When A. belladonna hairy roots were exposed to a high concentration of SA, AbSAMT1 mRNA begins to be expressed 12 h after the exposure, and steady expression continued over 144 h.
在颠茄毛状根中，外源添加的水杨酸（SA）通过该反应转化为水杨酸甲酯（MSA），该反应可能由S-腺苷-L-甲硫氨酸：水杨酸羧基甲基转移酶（SAMT）催化。在此，我们克隆了颠茄SAMT（AbSAMT1）的cDNA，其由357个氨基酸残基组成。它在大肠杆菌中表达，重组AbSAMT1表现出SAMT活性。当颠茄毛状根暴露于高浓度SA时，AbSAMT1 mRNA在暴露后12小时开始表达，并在144小时内持续稳定表达。
Zotero 插件