Moore H. M., Nasrallah J. B.
Section of Plant Biology, Division of Biological Sciences, Cornell University, Ithaca, New York 14853.
Plant Cell. 1990 Jan;2(1):29-38. doi: 10.1105/tpc.2.1.29.
Tobacco was transformed with a gene coding for an S-locus-specific glycoprotein of Brassica oleracea. The resulting transgenic plants showed tissue-specific and developmentally regulated expression of the introduced gene. Immunolocalization experiments showed that the Brassica gene was expressed in the stylar transmitting tissue of the transgenic plants. The pattern of expression of the introduced gene was more similar to that of the S-associated genes of Nicotiana alata than to expression in Brassica. Self-incompatibility was not conferred by the introduced gene.
用编码甘蓝S位点特异性糖蛋白的基因转化烟草。所得转基因植物显示出导入基因的组织特异性和发育调控表达。免疫定位实验表明,甘蓝基因在转基因植物的花柱传递组织中表达。导入基因的表达模式与烟草的S相关基因更相似,而与甘蓝中的表达不同。导入基因并未赋予自交不亲和性。