芸苔属S蛋白在转基因烟草雌蕊中沿花粉管路径积累于细胞间基质中。
Brassica S-Proteins Accumulate in the Intercellular Matrix along the Path of Pollen Tubes in Transgenic Tobacco Pistils.
作者信息
Kandasamy M. K., Dwyer K. G., Paolillo D. J., Doney R. C., Nasrallah J. B., Nasrallah M. E.
机构信息
Section of Plant Biology, Division of Biological Sciences, Cornell University, Ithaca, New York 14853.
出版信息
Plant Cell. 1990 Jan;2(1):39-49. doi: 10.1105/tpc.2.1.39.
Abstract
A tobacco plant transformed with a Brassica oleracea SLG-22 gene was analyzed by immunocytochemical methods to determine the localization of the transgene-encoded protein product. Immunolabeling was observed in the pistil along the path followed by pollen tubes after pollination. S-antigen accumulated in the intercellular matrix of the transmitting tissue of the style and its continuation in the basal portion of the stigma and outside a few special cells of the placental epidermis of the ovary. This pattern of S-antigen distribution closely resembles that described for the S-associated glycoproteins of self-incompatible Nicotiana alata and differs from its distribution in B. oleracea.
摘要
通过免疫细胞化学方法分析了用甘蓝SLG-22基因转化的烟草植株，以确定转基因编码的蛋白质产物的定位。授粉后，在雌蕊中沿着花粉管生长的路径观察到免疫标记。S抗原积累在花柱传递组织的细胞间基质中，以及在柱头基部的延续部分和子房胎盘表皮的一些特殊细胞外部。这种S抗原分布模式与自交不亲和的烟草中描述的S相关糖蛋白的分布模式非常相似，并且与其在甘蓝中的分布不同。
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