新城疫病毒V4株在马迪尔-德比牛肾细胞中的增殖。
Multiplication of the V4 strain of Newcastle disease virus in Madin Derby bovine kidney cells.
作者信息
Sahle M, Burgess W G, Kidanemariam A
机构信息
National Animal Health Research Center, Sebeta, Ethiopia.
出版信息
Onderstepoort J Vet Res. 2002 Sep;69(3):201-6.
PMID:12356166
Abstract
This study describes a reproducible cell culture system that permits the growth and secondary multiplication of the V4 strain of Newcastle disease virus. Allantoic fluid, magnesium chloride and diethylaminoethyl dextran were incorporated in Dulbecco's modified Eagle's medium to encourage secondary viral multiplication without adversely affecting healthy Madin Derby bovine kidney cell growth.
摘要
本研究描述了一种可重复的细胞培养系统，该系统可使新城疫病毒V4株生长并进行二次增殖。在杜氏改良 Eagle 培养基中加入尿囊液、氯化镁和二乙氨基乙基葡聚糖，以促进病毒二次增殖，同时不会对健康的麦迪逊-达比牛肾细胞生长产生不利影响。
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