Hiroi Noriko, Ito Takaaki, Yamamoto Hanako, Ochiya Takahiro, Jinno Shigeki, Okayama Hiroto
Department of Biochemistry and Molecular Biology, Graduate School of Medicine, The University of Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
EMBO J. 2002 Oct 1;21(19):5235-44. doi: 10.1093/emboj/cdf521.
Rcd1, initially identified as a factor essential for the commitment to nitrogen starvation-invoked differentiation in fission yeast, is one of the most conserved proteins found across eukaryotes, and its mammalian homolog is expressed in a variety of differentiating tissues. Here we show that mammalian Rcd1 is a novel transcriptional cofactor and is critically involved in the commitment step in the retinoic acid-induced differentiation of F9 mouse teratocarcinoma cells, at least in part, via forming complexes with retinoic acid receptor and activation transcription factor-2 (ATF-2). In addition, antisense oligonucleotide treatment of embryonic mouse lung explants suggests that Rcd1 also plays a role in retinoic acid-controlled lung development.
Rcd1最初被鉴定为裂殖酵母中氮饥饿诱导分化所必需的一个因子,是在真核生物中发现的最保守的蛋白质之一,其哺乳动物同源物在多种分化组织中表达。在此我们表明,哺乳动物Rcd1是一种新型转录辅因子,至少部分地通过与视黄酸受体和激活转录因子-2(ATF-2)形成复合物,在视黄酸诱导的F9小鼠畸胎瘤细胞分化的起始步骤中起关键作用。此外,对胚胎小鼠肺外植体进行反义寡核苷酸处理表明,Rcd1在视黄酸控制的肺发育中也发挥作用。