Bhandari Mohit, Schemitsch Emil H
Department of Clinical Epidemiology and Biostatistics, McMaster University Health Sciences Centre, Hamilton, Ontario, Canada.
J Bone Joint Surg Br. 2002 Sep;84(7):1054-61. doi: 10.1302/0301-620x.84b7.12532.
High-pressure lavage produces greater visible damage to bone at a macroscopic and microscopic level when compared with low-pressure lavage and can result in delay in the healing of fractures. Osteoblasts and adipocytes are derived from mesenchymal stem cells. Conditions which lead to bone loss often involve a switch from the osteoblast to adipocyte lineage. We have therefore examined the effect of high- and low-pressure irrigation on the differentiation of adipocytes. Calvaria-derived bone cells were exposed to either low-pressure or high-pressure irrigation with normal saline. After 14 days the cells were fixed and the osteoblasts and adipocytes quantified using Oil Red O to stain cytoplasmic lipid droplets (triglycerides) in the cells. Osteoblasts were quantified using a commercially available alkaline-phosphatase staining assay. A standard quantitative reverse transcription-polymerase chain reaction (RT-PCR) was performed. Messenger RNA levels for osteocalcin, a marker of osteoblasts, and PPARy2, a marker of adipocytes, were measured. High-pressure lavage resulted in an increase in adipogenesis of 50% when compared with low-pressure lavage. Our findings suggest that high-pressure lavage may promote differentiation of mesenchymal stem cells towards the adipocyte lineage. This may have clinical significance in the development of delayed and nonunion after treatment of fractures of long bones.
与低压灌洗相比，高压灌洗在宏观和微观层面上对骨骼造成的可见损伤更大，并且可能导致骨折愈合延迟。成骨细胞和脂肪细胞均来源于间充质干细胞。导致骨质流失的情况通常涉及从成骨细胞谱系向脂肪细胞谱系的转变。因此，我们研究了高压和低压灌洗对脂肪细胞分化的影响。将取自颅骨的骨细胞用生理盐水进行低压或高压灌洗。14天后，将细胞固定，并用油红O对细胞内的细胞质脂滴（甘油三酯）进行染色，以对成骨细胞和脂肪细胞进行定量分析。使用市售的碱性磷酸酶染色法对成骨细胞进行定量分析。进行了标准的定量逆转录-聚合酶链反应（RT-PCR）。检测了成骨细胞标志物骨钙素和成脂细胞标志物PPARy2的信使核糖核酸水平。与低压灌洗相比，高压灌洗使脂肪生成增加了50%。我们的研究结果表明，高压灌洗可能会促进间充质干细胞向脂肪细胞谱系分化。这可能对长骨骨折治疗后延迟愈合和不愈合的发生具有临床意义。
