Przywarska-Boniecka H, Trynda L, Antonini E
Eur J Biochem. 1975 Apr 1;52(3):567-73. doi: 10.1111/j.1432-1033.1975.tb04028.x.
The reaction between iron and cobalt tetrasulfonated phthalocyanines and globin results in the formation of the green complexes, as has been proved by difference spectroscopy. Spectrophotometric titration data indicate the formation of those complexes at the molar ratio 1:1. The complexes of ferrous, ferric and cobaltous tetrasulfonated phthalocyanines with globin have been isolated from the reaction mixtures by separation on Sephadex G-50 and precipitation of the protein fractions with ammonium sulfate. The visible spectra of these complexes are characterised by the main intensive peak at 641 nm, 678 nm, and 675 nm for ferric, ferrous and cobaltous derivatives, respectively. The new globin complexes have the property of reversible combination with oxygen and coordination with cyanide ions. It is evidence from the results of the spectrophotometric titrations of hemoglobin and methemoglobin with cobaltous tetrasulfonated phthalocyanine that iron protoporphyrins are displaced by this cobalt derivative; this suggests that phthalocyanine and porphyrin are bonded in a similar manner.
铁与四磺化酞菁钴和球蛋白之间的反应会生成绿色络合物，这已通过差示光谱法得到证实。分光光度滴定数据表明这些络合物以1:1的摩尔比形成。亚铁、铁和亚钴四磺化酞菁与球蛋白的络合物已通过在Sephadex G - 50上的分离以及用硫酸铵沉淀蛋白质组分从反应混合物中分离出来。这些络合物的可见光谱的特征在于，对于铁、亚铁和亚钴衍生物，分别在641 nm、678 nm和675 nm处有主要的强峰。新的球蛋白络合物具有与氧可逆结合以及与氰离子配位的性质。用亚钴四磺化酞菁对血红蛋白和高铁血红蛋白进行分光光度滴定的结果表明，这种钴衍生物会取代铁原卟啉；这表明酞菁和卟啉以类似的方式结合。
