Sharakhov Igor V, Serazin Andrew C, Grushko Olga G, Dana Ali, Lobo Neil, Hillenmeyer Maureen E, Westerman Richard, Romero-Severson Jeanne, Costantini Carlo, Sagnon N'Fale, Collins Frank H, Besansky Nora J
Center for Tropical Disease Research and Training, University of Notre Dame, Notre Dame, IN 46556-0369, USA.
Science. 2002 Oct 4;298(5591):182-5. doi: 10.1126/science.1076803.
In tropical Africa, Anopheles funestus is one of the three most important malaria vectors. We physically mapped 157 A. funestus complementary DNAs (cDNAs) to the polytene chromosomes of this species. Sequences of the cDNAs were mapped in silico to the A. gambiae genome as part of a comparative genomic study of synteny, gene order, and sequence conservation between A. funestus and A. gambiae. These species are in the same subgenus and diverged about as recently as humans and chimpanzees. Despite nearly perfect preservation of synteny, we found substantial shuffling of gene order along corresponding chromosome arms. Since the divergence of these species, at least 70 chromosomal inversions have been fixed, the highest rate of rearrangement of any eukaryote studied to date. The high incidence of paracentric inversions and limited colinearity suggests that locating genes in one anopheline species based on gene order in another may be limited to closely related taxa.
在热带非洲，嗜人按蚊是三种最重要的疟疾传播媒介之一。我们将157个嗜人按蚊互补DNA（cDNA）物理定位到该物种的多线染色体上。作为对嗜人按蚊和冈比亚按蚊之间的同线性、基因顺序和序列保守性进行比较基因组研究的一部分，这些cDNA序列在计算机上被定位到冈比亚按蚊基因组。这两个物种属于同一亚属，其分化时间与人类和黑猩猩的分化时间相近。尽管同线性几乎完美保存，但我们发现沿相应染色体臂的基因顺序发生了大量重排。自这些物种分化以来，至少70次染色体倒位已被固定，这是迄今为止所研究的任何真核生物中最高的重排率。臂内倒位的高发生率和有限的共线性表明，基于另一种按蚊的基因顺序在一种按蚊中定位基因可能仅限于密切相关的分类群。
