Gupta R S, Sharma Rakhi, Sharma Aruna, Bhatnager A K, Dobhal M P, Joshi Y C, Sharma M C
Reproduction Physiology Section, Department of Zoology, University of Rajasthan, Jaipur-302004, India.
Asian J Androl. 2002 Sep;4(3):175-8.
To evaluate the antifertility effect of Alstonia scholaris bark extract in male rats.
In male Wistar rats Alstonia scholaris bark extract was given by oral route at a dose of 200 mg/day for 60 days. The fertility and testicular function were assessed by mating tests, sperm motility, sperm concentration, biochemical indices and testicular cell population dynamics.
Oral feeding with the extract at a dose of 200 mg/day for the period of 60 days did not cause body weight loss, while the weights of testes, epididymides, seminal vesicle and ventral prostate were significantly reduced. The production of step-19 spermatids was reduced by 79.6% in treated rats. The population of preleptotene and pachytene spermatocytes were decreased by 61.9% and 60.1%, respectively. Spermatogonia and Sertoli cell population were also affected. The seminiferous tubule and Leydig cell nuclear area were reduced significantly (P<0.01) when compared to the controls. Reduced sperm count and motility resulted in a total suppression of fertility. A significant fall in the protein and sialic acid content of the testes, epididymides, seminal vesicle and ventral prostate as well as glycogen content of testes were also noticed. The fructose content in the seminal vesicle was lowered whereas the testicular cholesterol was elevated as compared with the controls. The following compounds were obtained from the extract with chromatographic separation over Si-gel column: agr-amyrin, bgr-amyrin, lupiol acetate, venenative, rhazine and yohimbine.
At the dose level employed, Alstonia scholaris bark extract has a significant antifertility effect in male rats; the primary site of action may be post meiotic germ cells (Step 19 spermatids).
评估印度萝芙木树皮提取物对雄性大鼠的抗生育作用。
对雄性Wistar大鼠经口给予印度萝芙木树皮提取物，剂量为200毫克/天，持续60天。通过交配试验、精子活力、精子浓度、生化指标和睾丸细胞群体动态评估生育力和睾丸功能。
以200毫克/天的剂量经口喂食提取物60天未导致体重减轻，但睾丸、附睾、精囊和腹侧前列腺的重量显著降低。处理组大鼠中第19步精子细胞的产生减少了79.6%。细线前期和粗线期精母细胞的数量分别减少了61.9%和60.1%。精原细胞和支持细胞群体也受到影响。与对照组相比，曲细精管和间质细胞核面积显著减小（P<0.01）。精子数量和活力降低导致生育力完全抑制。还观察到睾丸、附睾、精囊和腹侧前列腺的蛋白质和唾液酸含量以及睾丸糖原含量显著下降。与对照组相比，精囊中果糖含量降低，而睾丸胆固醇升高。通过硅胶柱色谱分离从提取物中获得了以下化合物：α-香树脂醇、β-香树脂醇、乙酸羽扇豆醇酯、毒毛旋花子甙元、利血平宁和育亨宾。
在所采用的剂量水平下，印度萝芙木树皮提取物对雄性大鼠具有显著的抗生育作用；主要作用部位可能是减数分裂后的生殖细胞（第19步精子细胞）。
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