合欢树皮提取物对雄性白化大鼠的抗生精和抗雄激素活性。

Antispermatogenic, antiandrogenic activities of Albizia lebbeck (L.) Benth bark extract in male albino rats.

作者信息

Gupta R S, Kachhawa J B S, Chaudhary R

机构信息

Reproduction Physiology Section, Department of Zoology, University of Rajasthan, Jaipur 302 004, India.

出版信息

Phytomedicine. 2006 Mar;13(4):277-83. doi: 10.1016/j.phymed.2004.11.008. Epub 2005 Sep 19.

DOI:10.1016/j.phymed.2004.11.008

PMID:16492532

Abstract

Methanolic extract of Albizia lebbeck bark when administered orally at the dose level of 100 mg/rat/day to male rats of proven fertility for 60 days did not cause any significant loss in their body weights but the weights of reproductive organs, i.e. testis, epididymides, seminal vesicle and ventral prostate were decreased in a significant manner when compared to controls. Sperm motility as well as sperm density were reduced significantly which resulted in reduction of male fertility by 100%. Marked decline in the germ cell population was noticed. Population of preleptotene, pachytene, secondary spermatocytes and step-19 spermatid were declined by 60.86%, 65.81%, 71.56% and 66.55%, respectively. Cross-sectional surface area of sertoli cells as well as the cells counts were found to be depleted significantly. Leydig cells nuclear area and number of mature Leydig cells were decreased by 60.03% and 51.56%, respectively. Serum testosterone levels showed significant reduction after A. lebbeck extract feeding. Oral administration of the extract did not affect red blood cell (RBC) and white blood cell (WBC) count, haemoglobin, haematocrit and glucose in the blood and cholesterol, protein, triglyceride and phospholipid in the serum. In conclusion, A. lebbeck bark extract administration arrests spermatogenesis in male rats without noticeable side effects.

摘要

将阿勒勃树皮的甲醇提取物以100毫克/大鼠/天的剂量口服给予经证实具有生育能力的雄性大鼠，持续60天，大鼠体重未出现任何显著下降，但与对照组相比，其生殖器官，即睾丸、附睾、精囊和腹侧前列腺的重量显著降低。精子活力和精子密度均显著降低，导致雄性生育能力下降100%。观察到生殖细胞数量明显减少。细线前期、粗线期、次级精母细胞和19期精子细胞的数量分别下降了60.86%、65.81%、71.56%和66.55%。支持细胞的横截面积和细胞数量均显著减少。睾丸间质细胞核面积和成熟睾丸间质细胞数量分别减少了60.03%和51.56%。给予阿勒勃提取物后，血清睾酮水平显著降低。口服该提取物对血液中的红细胞（RBC）和白细胞（WBC）计数、血红蛋白、血细胞比容和血糖以及血清中的胆固醇、蛋白质、甘油三酯和磷脂均无影响。总之，给予阿勒勃树皮提取物可使雄性大鼠精子发生停滞，且无明显副作用。