Kim So Hee, Moon Young Jin, Ryu Chung-Kyu, Lee Myung Gull
College of Dentistry and Research Institute of Oral Science, Kangnung National University, 123, Jibyeon-Dong, Gangnung, Gangwon-Do 210-702, South Korea.
J Pharm Biomed Anal. 2002 Oct 15;30(3):519-26. doi: 10.1016/s0731-7085(02)00398-9.
A high-performance liquid chromatographic (HPLC) method was developed for the determination of a new isoquinolinedione derivative, 7-anilino-5,8-isoquinolinedione (IQO4), in rat plasma, urine, blood and tissue homogenates using diazepam as an internal standard. A 2 volume of acetonitrile was added to deproteinize the biological sample. A 50 microl aliquot of the supernatant was injected onto a C(18) reversed-phase column. The mobile phase, 0.05 M acetate buffer (pH 3):acetonitrile:methanol (40:40:20, v/v/v), was run at a flow rate of 1.5 ml/min. The column effluent was monitored using an ultraviolet detector set at 298 nm. The retention times for IQO4 and the internal standard were approximately 5 and 7 min, respectively. The detection limits of IQO4 in rat plasma, urine and tissue homogenates (including blood) were 0.05, 0.1 and 0.1 microg/ml, respectively. The coefficients of variation of the assay were below 9.4% for rat plasma, urine and tissue homogenates. No interferences from endogenous substances were found.
建立了一种高效液相色谱（HPLC）法，以地西泮为内标，用于测定大鼠血浆、尿液、血液和组织匀浆中的新型异喹啉二酮衍生物7-苯胺基-5,8-异喹啉二酮（IQO4）。加入2倍体积的乙腈使生物样品脱蛋白。取50微升上清液注入C(18)反相柱。流动相为0.05 M醋酸盐缓冲液（pH 3）：乙腈：甲醇（40:40:20，v/v/v），流速为1.5 ml/min。使用设置在298 nm的紫外检测器监测柱流出物。IQO4和内标的保留时间分别约为5分钟和7分钟。IQO4在大鼠血浆、尿液和组织匀浆（包括血液）中的检测限分别为0.05、0.1和0.1微克/毫升。该测定法在大鼠血浆、尿液和组织匀浆中的变异系数低于9.4%。未发现内源性物质的干扰。
