Hammam Essam
Department of Chemistry, Faculty of Science, Tanta University, 31527 Tanta, Egypt.
J Pharm Biomed Anal. 2002 Oct 15;30(3):651-9. doi: 10.1016/s0731-7085(02)00344-8.
Nitrofurnation is an antibacterial drug. It is used in the treatment of initial or recurrent urinary tract infections caused by susceptible organisms. The cyclic voltammogram of the drug in Britton-Robinson buffers (pH 2-11) exhibited a single well-defined cathodic peak at the hanging mercury drop electrode, that due to the reduction of its nitro group to the amine stage. A fully validated, sensitive, and reproducible developed procedure was described for determination of the drug in bulk form, pharmaceutical formulation, human serum and human urine using, square-wave cathodic adsorptive stripping voltammetry. The optimal experimental parameters for the drug assay were: accumulation potential = -0.4 V (vs. Ag/AgCl/ KCl(s)), accumulation time = 40 s, frequency = 120 Hz, pulse amplitude = 50 mV and scan increment = 10 mV in Britton-Robinson buffer (pH 10). A mean percentage recovery of 100.68 +/- 0.17 (n = 5) and a detection limit of 1.32 x 10(-10) M of bulk drug were achieved. Applicability to assay of the drug in pharmaceutical formulation, human serum and human urine was studied and illustrated. The mean percentage recoveries were found as: 101.49 +/- 0.65, 103.94 +/- 0.73 and 101.98 +/- 0.52 (n = 5) in pharmaceutical formulation, human serum and human urine, respectively. Detection limits of 2.86 x 10(-10) M and 5.77 x 10(-10) M nitrofurantoin were achieved in human serum and urine, respectively.
呋喃妥因是一种抗菌药物。它用于治疗由易感微生物引起的初始或复发性尿路感染。该药物在 Britton-Robinson 缓冲液（pH 2 - 11）中的循环伏安图在悬汞滴电极上呈现出一个明确的阴极峰，这是由于其硝基还原为胺阶段所致。描述了一种经过充分验证、灵敏且可重现的方法，用于使用方波阴极吸附溶出伏安法测定原料药、药物制剂、人血清和人尿中的该药物。药物测定的最佳实验参数为：在 Britton-Robinson 缓冲液（pH 10）中，富集电位 = -0.4 V（相对于 Ag/AgCl/KCl(s)），富集时间 = 40 s，频率 = 120 Hz，脉冲幅度 = 50 mV，扫描增量 = 10 mV。原料药的平均回收率为 100.68 ± 0.17（n = 5），检测限为 1.32×10⁻¹⁰ M。研究并说明了该方法在药物制剂、人血清和人尿中药物测定的适用性。在药物制剂、人血清和人尿中的平均回收率分别为：101.49 ± 0.65、103.94 ± 0.73 和 101.98 ± 0.52（n = 5）。人血清和尿中呋喃妥因的检测限分别为 2.86×10⁻¹⁰ M 和 5.77×10⁻¹⁰ M。
