N-羟基吡啶-2-硫酮（奥美定）对L5178Y小鼠淋巴瘤细胞的细胞毒性和遗传毒性作用研究。

Studies on cytotoxic and genotoxic effects of N-hydroxypyridine-2-thione (Omadine) in L5178Y mouse lymphoma cells.

作者信息

Möller Marianne, Adam Waldemar, Saha-Möller Chantu R, Stopper Helga

机构信息

Institute of Pharmacology and Toxicology, University of Würzburg, Versbacherstrasse 9, D-97078 Würzburg, Germany.

出版信息

Toxicol Lett. 2002 Nov 15;136(1):77-84. doi: 10.1016/s0378-4274(02)00279-5.

DOI:10.1016/s0378-4274(02)00279-5

PMID:12368059

Abstract

The cytotoxicity and genotoxicity of the antifungal and antimicrobial agent Omadine, i.e. N-hydroxypyridine-2-thione (HOPT), has been investigated in L5178Y mouse lymphoma cells in the dark and under UVA irradiation. Omadine inhibits cell growth and induces micronuclei at concentrations >0.5 microM in the absence of light. At a 0.5-microM concentration, an UVA-dose-dependent induction of micronuclei is observed, conditions at which the cytotoxicity and genotoxicity in the dark is negligible. The photogenotoxicity is not accompanied by cytotoxicity. Control experiments with the radical scavengers GSH and GSHOEt implicate the involvement of hydroxyl radicals in the photogenotoxicity of Omadine.

摘要

抗真菌和抗菌剂奥麦丁（即N-羟基吡啶-2-硫酮，HOPT）的细胞毒性和遗传毒性已在L5178Y小鼠淋巴瘤细胞中于黑暗及UVA照射条件下进行了研究。在无光条件下，奥麦丁在浓度>0.5微摩尔时会抑制细胞生长并诱导微核形成。在0.5微摩尔浓度下，观察到微核的UVA剂量依赖性诱导，而在这些条件下黑暗中的细胞毒性和遗传毒性可忽略不计。光遗传毒性并不伴有细胞毒性。用自由基清除剂谷胱甘肽（GSH）和GSHOEt进行的对照实验表明，羟基自由基参与了奥麦丁的光遗传毒性。