体外微核试验中TK6人淋巴母细胞和L5178Y小鼠淋巴瘤细胞系的比较研究

A comparative study of TK6 human lymphoblastoid and L5178Y mouse lymphoma cell lines in the in vitro micronucleus test.

作者信息

Zhang L S, Honma M, Hayashi M, Suzuki T, Matsuoka A, Sofuni T

机构信息

Division of Genetics and Mutagenesis, National Institute of Health Sciences, Tokyo, Japan.

出版信息

Mutat Res. 1995 Aug;347(3-4):105-15. doi: 10.1016/0165-7992(95)00027-5.

DOI:10.1016/0165-7992(95)00027-5

PMID:7565900

Abstract

Micronucleus induction was compared in human lymphoblastoid TK6 and mouse lymphoma L5178Y cell lines treated with model clastogens and spindle poisons, i.e., X-rays, methyl methanesulfonate, ethyl methanesulfonate, mitomycin C, colcemid, and vincristine. The spontaneous micronucleated cell (MNC) frequency was stable and reproducible in both cell lines. All clastogens and spindle poisons studied here induced micronuclei in both cell lines. They increased MNC frequency at lower concentrations or caused a greater increase at the same concentration in TK6 cells. These clastogens and spindle poisons, however, were also more toxic to TK6 than to L5178Y cells and when comparison was based on cytotoxicity, they showed more efficient MNC induction in L5178Y cells. In conclusion, neither cell line was superior to the other, and both of them can be used as target cells in the in vitro micronucleus assay.

摘要

在用模型断裂剂和纺锤体毒物（即X射线、甲基磺酸甲酯、乙基磺酸甲酯、丝裂霉素C、秋水仙酰胺和长春新碱）处理的人淋巴母细胞TK6和小鼠淋巴瘤L5178Y细胞系中比较了微核诱导情况。两种细胞系中自发微核细胞（MNC）频率稳定且可重复。此处研究的所有断裂剂和纺锤体毒物均在两种细胞系中诱导微核形成。它们在较低浓度下增加了MNC频率，或在相同浓度下使TK6细胞中的增加幅度更大。然而，这些断裂剂和纺锤体毒物对TK6的毒性也比对L5178Y细胞的毒性更大，当基于细胞毒性进行比较时，它们在L5178Y细胞中显示出更有效的MNC诱导作用。总之，两种细胞系都不优于另一种，并且它们都可以用作体外微核试验中的靶细胞。