Takahashi Yuichi, Nagoya Takao, Ohta Nobuo
The Yamagata Prefectural Institute of Public Health.
Arerugi. 2002 Aug;51(8):609-14.
After collection of airborne particles with a seven-day recording volumetric spore trap (Burkard model) using optically clear, pressure sensitive acrylic adhesive tapes, a dry PVDF membrane was pressed down firmly onto the adhesive tape, antigen-antibody reaction was performed in full contact with the tapes and PVDF membrane. Dark purple spots from airborne pollen allergens were examined under a light microscope to evaluate the form of pollens and particles wit h the antigenicity. It is clarified that identification of the form of pollens with the antigencity is possible not only pollens with the antigenicity but a pollen has already lost its shape, and it is also clarified that some airborne particular matters have the pollen antigencity. Airborne samples were collected during the Cryptomeria japonica or grass pollen season. Samples collected during the C. japonica pollen season were treated with anti-Cry j 1 monoclonal antibody, and those during the grass pollen season with anti-Dac g rabbit IgG. Samples collected during the C. japonica and the grass pollen peak season were treated with antibodies from sera of pollinosis patients. Spots originated not only from relevant pollen grains but also from parts of airborne particular matter have the pollen antigenicity and some spots could not observed for any kind of particles.
使用光学透明的压敏丙烯酸胶带，通过七天记录容量的孢子捕捉器（伯卡德模型）收集空气中的颗粒后，将干燥的聚偏二氟乙烯（PVDF）膜牢固地压在胶带上，使抗原 - 抗体反应与胶带和PVDF膜完全接触。在光学显微镜下检查空气中花粉过敏原产生的深紫色斑点，以评估花粉和具有抗原性的颗粒的形态。结果表明，不仅可以识别具有抗原性的花粉形态，对于已经失去形状的花粉也可以识别，还表明一些空气中的颗粒物具有花粉抗原性。在日本柳杉或草花粉季节收集空气样本。在日本柳杉花粉季节收集的样本用抗Cry j 1单克隆抗体处理，在草花粉季节收集的样本用抗Dac g兔IgG处理。在日本柳杉和草花粉高峰期收集的样本用花粉症患者血清中的抗体处理。不仅来自相关花粉粒，而且来自空气中颗粒物部分的斑点都具有花粉抗原性，并且有些斑点在任何类型的颗粒中都未观察到。
