Lomholt Bodil, Christensen Knud, Frederiksen Sune
Department of Medical Biochemistry and Genetics, Biochemistry Laboratory B, Panum Institute, University of Copenhagen, Blegdamsvej 3, DK-2200 Copenhagen N. Denmark.
Hereditas. 2002;136(2):104-7. doi: 10.1034/j.1601-5223.2002.1360203.x.
A karyotype for the guinea pig (Cavio cambayo) is proposed based on an R-banding pattern. R-bands were obtained by BrdU incorporation into the cells followed by a combined DAPI and propidium iodide staining of the fixed metaphase spreads. In situ hybridization was performed with two biotinylated 18-mer PNA (peptide nucleic acid) probes complementary to sequences within the 5S rRNA gene. The 5S rRNA gene repeats map to chromosomes 7q2. 20q2 and 30q2, respectively.