Jayasagar G, Kumar M Krishna, Chandrasekhar K, Prasad P Siva, Rao Y Madhusudan
Centre for Bio-Pharmaceutics and Pharmacokinetics, University College of Pharmaceutical Sciences, Kakatiya University, Warangal, Andhra Pradesh, India.
Pharmazie. 2002 Sep;57(9):619-21.
A simple high performance liquid chromatographic method using UV detection for the determination of celecoxib, a specific COX 2 inhibitor, in serum was developed. Serum samples containing the internal standard, tolbutamide, are eluted through a C18, Wakosil column. After extracting with dichloromethane, the eluent is monitored at 250 nm. The mobile phase comprised of 10 mM potassium dihydrogen ortho phosphate (pH 3.2) and acetonitrile (50:50 v/v) with a flow rate of 1 ml/min. Retention times of celecoxib and tolbutamide were 9.6 and 3.5 min, respectively. The mean absolute recovery value was about 70-80%, while the intra day and inter day coefficient of variation and percent error values of the assay method were less than 10%. The calibration curve was linear over a concentration range of 10-1000 ng/ml.
建立了一种使用紫外检测的简单高效液相色谱法，用于测定血清中特异性COX 2抑制剂塞来昔布。含有内标甲苯磺丁脲的血清样品通过Wakosil C18柱洗脱。用二氯甲烷萃取后，在250 nm处监测洗脱液。流动相由10 mM磷酸二氢钾（pH 3.2）和乙腈（50:50 v/v）组成，流速为1 ml/min。塞来昔布和甲苯磺丁脲的保留时间分别为9.6和3.5分钟。平均绝对回收率约为70-80%，而该测定方法的日内和日间变异系数及误差百分比值均小于10%。校准曲线在10-1000 ng/ml的浓度范围内呈线性。
