Góźdź A, Szczepańska-Sadowska E, Szczepańska K, Maśliński W, Luszczyk B
Department of Experimental and Clinical Physiology, Medical University of Warsaw, Poland.
J Physiol Pharmacol. 2002 Sep;53(3):349-57.
Vasopressin plays significant role in regulation of blood pressure by means of V1 and V2 receptors, however regulation of synthesis of these receptors in hypertension is only poorly recognized. The purpose of the present study was to compare expression of V1a, V1b and V2 vasopressin (R) mRNA in the renal cortex, renal medulla and the heart of hypertensive renin transgenic TGR(mRen2)27 rats (TGR) and of their parent normotensive Sprague Dawley (SD) strain. The study was performed on 12 weeks old TGR and SD rats. Competitive PCR method was used for quantitative analysis of V1a, V1b and V2 receptors mRNA in fragments of renal cortex, renal medulla and apex of the left ventricle of the heart. In both strains expression of V1aR and V2R mRNA was significantly greater in the renal medulla than in the renal cortex. In the renal medulla but not in the cortex expression of V1aR mRNA was significantly greater in TGR than in SD rats. V2R mRNA expression was similar in the renal cortex and renal medulla of both strains. V1aR mRNA was well expressed in the heart of SD and TGR rats, however there was no significant difference between these two strains. V2R mRNA was not present in the heart. V1bR mRNa could not be detected either in the kidney or in the heart. The results provide evidence for specific increase of expression of V1a receptors mRNA in the renal medulla of TGR rats.
血管加压素通过V1和V2受体在血压调节中发挥重要作用，然而高血压状态下这些受体合成的调节机制却鲜为人知。本研究旨在比较高血压肾素转基因TGR（mRen2）27大鼠（TGR）及其正常血压的亲代斯普拉格-道利（SD）品系大鼠的肾皮质、肾髓质和心脏中V1a、V1b和V2血管加压素（R）mRNA的表达情况。研究对象为12周龄的TGR大鼠和SD大鼠。采用竞争性PCR方法对肾皮质、肾髓质和心脏左心室顶端片段中的V1a、V1b和V2受体mRNA进行定量分析。在两个品系中，肾髓质中V1aR和V2R mRNA的表达均显著高于肾皮质。在肾髓质而非皮质中，TGR大鼠的V1aR mRNA表达显著高于SD大鼠。两个品系的肾皮质和肾髓质中V2R mRNA表达相似。V1aR mRNA在SD大鼠和TGR大鼠的心脏中均有良好表达，但两个品系之间无显著差异。心脏中未检测到V2R mRNA。在肾脏和心脏中均未检测到V1bR mRNa。结果表明TGR大鼠肾髓质中V1a受体mRNA表达特异性增加。
