Yegin A, Akbas S H, Ozben T, Korgun D K
Department of Biochemistry, Faculty of Medicine, Akdeniz University, Antalya, Turkey.
Acta Neurol Scand. 2002 Nov;106(5):258-62. doi: 10.1034/j.1600-0404.2002.01238.x.
Previous studies have revealed an increase in several forms of phospholipase A2 activity associated with cell injury, but the secretory form of phospholipase A2 has not previously been studied in neurological disorders. We investigated the influence of seizures on secretory phospholipase A2 and phospholipid breakdown in synaptosome fractions prepared from rat hippocampus, cortex and cerebellum in pentylenetetrazol-induced epilepsy.
Secretory phospholipase A2 concentration was measured by a photometric enzyme immunoassay. The synaptosomes underwent extraction, and the phospholipids fractions for phosphatidylcholine, phosphatidylethanolamine and lysophosphatidylcholine were recovered from the thin layer chromatography plates. The amount of each phospholipid was quantified using the amount of recovered phosphate in each phospholipid spot.
Secretory phospholipase A2 concentration was found to be significantly higher in the epileptic group when compared with the control group. The amounts of phospholipids were found to be highly variable in different brain regions.
Our results suggest that epileptic seizures enhanced phospholipid breakdown and induced alterations in the distribution of phospholipids in different brain regions.
以往研究显示，与细胞损伤相关的多种磷脂酶A2活性有所增加，但此前尚未在神经疾病中对分泌型磷脂酶A2进行研究。我们研究了癫痫发作对由戊四氮诱导癫痫的大鼠海马体、皮质和小脑中制备的突触体组分中分泌型磷脂酶A2及磷脂分解的影响。
采用光度酶免疫分析法测定分泌型磷脂酶A2浓度。对突触体进行提取，从薄层色谱板上回收磷脂酰胆碱、磷脂酰乙醇胺和溶血磷脂酰胆碱的磷脂组分。利用每个磷脂斑点中回收的磷酸盐量对每种磷脂的量进行定量。
发现癫痫组的分泌型磷脂酶A2浓度显著高于对照组。不同脑区的磷脂含量差异很大。
我们的结果表明，癫痫发作增强了磷脂分解，并导致不同脑区磷脂分布发生改变。
