Obal I, Siklós L, Engelhardt J I
Department of Neurology, University of Szeged, Szeged, Hungary.
Acta Neurol Scand. 2002 Nov;106(5):282-91. doi: 10.1034/j.1600-0404.2002.01252.x.
The effect of IgG from patients with multifocal motor neuropathy (MMN) on the content and distribution of calcium in spinal motoneurons was compared with the effect of IgG from patients with sporadic amyotrophic lateral sclerosis (SALS) and IgG from normal individuals.
Different purified IgG samples were injected intraperitoneally in mice. Then, the animals were subjected to histochemical techniques to visualize calcium in electron microscopic sections.
Quantitative morphometric analysis verified that IgG from MMN decreased the vesicular and axoplasmic calcium content in the axon terminals at the neuromuscular junctions and had no influence on the perikaryon. In contrast to this, IgG from patients with SALS increased the intracellular calcium both in the axon terminal and in the perikaryon. IgG from normal individuals exerted no effect. Elevated intracellular calcium may contribute to motoneuron degeneration. The lack of such effect with MMN immunoglobulins helps to explain the relative sparing of motoneurons in the disease.
将多灶性运动神经病（MMN）患者的IgG对脊髓运动神经元中钙含量及分布的影响，与散发性肌萎缩侧索硬化症（SALS）患者的IgG及正常个体的IgG的影响进行比较。
将不同的纯化IgG样本腹腔注射到小鼠体内。然后，对动物进行组织化学技术处理，以在电子显微镜切片中观察钙。
定量形态计量分析证实，MMN患者的IgG降低了神经肌肉接头处轴突终末的囊泡和轴浆钙含量，且对胞体无影响。与此相反，SALS患者的IgG增加了轴突终末和胞体内的细胞内钙。正常个体的IgG无作用。细胞内钙升高可能导致运动神经元变性。MMN免疫球蛋白缺乏这种作用有助于解释该疾病中运动神经元相对保留的现象。
