Wessagowit V, South A P
Department of Cell and Molecular Pathology, St John's Institute of Dermatology, The Guy's, King's College and St Thomas' Hospitals' Medical School, St Thomas' Hospital, London, UK.
Clin Exp Dermatol. 2002 Sep;27(6):485-92. doi: 10.1046/j.1365-2230.2002.01117.x.
The Human Genome Project and other large scale sequencing consortia continue to generate huge amounts of DNA sequence data. Despite the identification of specific disease-related genes and genetic markers, we still appear to know little about how diverse gene products actually interact with each other or respond to other chemical or biological stimuli. Such information is of course fundamental to understanding complex disease pathways and biochemical processes and, as such, has spawned new fields of investigative genetics, that of functional genomics and proteomics. DNA array technology is emerging as a powerful, high-throughput and versatile tool that can be applied to the study of functional genomics. This article reviews the methodology involved in array analysis and provides insight into how, as an investigative tool, DNA arrays are becoming increasingly useful in understanding fundamental abnormalities in dermatological disease and also in refining the management of patients with certain skin disorders.
人类基因组计划和其他大规模测序联盟持续产生海量的DNA序列数据。尽管已鉴定出特定的疾病相关基因和遗传标记，但我们似乎仍对各种基因产物如何实际相互作用或对其他化学或生物刺激作出反应知之甚少。此类信息对于理解复杂的疾病途径和生化过程至关重要，正因如此，催生了新的研究遗传学领域，即功能基因组学和蛋白质组学。DNA阵列技术正成为一种强大、高通量且通用的工具，可应用于功能基因组学研究。本文回顾了阵列分析所涉及的方法，并深入探讨了作为一种研究工具，DNA阵列如何在理解皮肤病学疾病的基本异常以及优化某些皮肤疾病患者的管理方面变得越来越有用。
