Iwafune Mito, Kakizaki Ikuko, Yukawa Masahiro, Kudo Daisuke, Ota Sakae, Endo Masahiko, Takagaki Keiichi
Department of Biochemistry, Hirosaki University School of Medicine, 5 Zaifu-cho, 036-8562, Hirosaki, Japan.
Biochem Biophys Res Commun. 2002 Oct 11;297(5):1167-70. doi: 10.1016/s0006-291x(02)02350-1.
The glycosaminoglycan chain of decorin from human spinal ligaments was digested using the hydrolysis of bovine testicular hyaluronidase. As a result, decorin with hexasaccharide, octasaccharide, and decasaccharide including the linkage region, GlcA-Gal-Gal-Xyl, was obtained. The obtained decorin as an acceptor and hyaluronic acid as a donor were incubated with bovine testicular hyaluronidase under the condition of transglycosylation reaction. The transglycosylation reaction product had hexasaccharide to triacontasaccharide. Judging from the analysis of glycosaminoglycan chain in the transglycosylation reaction product, it was confirmed that hyaluronic acid chain as a donor was transferred to the retained glycosaminoglycan chain of decorin as an acceptor. Similarly, it was possible to reconstruct the glycosaminoglycan chain in decorin to chondroitin, chondroitin 4-sulfate or chondroitin 6-sulfate. Therefore, we succeeded in synthesizing an artificial family of decorins.
使用牛睾丸透明质酸酶水解法对人脊柱韧带中核心蛋白聚糖的糖胺聚糖链进行消化。结果，得到了包含连接区域GlcA-Gal-Gal-Xyl的六糖、八糖和十糖的核心蛋白聚糖。将得到的作为受体的核心蛋白聚糖和作为供体的透明质酸在转糖基化反应条件下与牛睾丸透明质酸酶一起孵育。转糖基化反应产物含有六糖至三十糖。从转糖基化反应产物中糖胺聚糖链的分析判断，证实作为供体的透明质酸链转移到了作为受体的核心蛋白聚糖保留的糖胺聚糖链上。同样，也可以将核心蛋白聚糖中的糖胺聚糖链重建成软骨素、硫酸软骨素4或硫酸软骨素6。因此，我们成功合成了人工核心蛋白聚糖家族。
