催产素与牛神经垂体素II的协同结合。
Co-operative binding of oxytocin to bovine neurophysin II.
作者信息
Hope D B, Wälti M, Winzor D J
出版信息
Biochem J. 1975 May;147(2):377-9. doi: 10.1042/bj1470377.
Abstract
The interaction of oxytocin with bovine neurophysin II in 0.1 M-sodium phosphate, pH 5.8, was investigated by equilibrium-dialysis and sedimentation studies. Sigmoidality of the binding curve is attributed to isomerization, either hormone-induced or pre-existing, with preferential binding of oxytocin to one isomeric state. Results are consistent with a binding equation of the form r = (2P[S]+2PQ[S]2)/(1+2P[S]+PQ[S]2) and values of 0.7 X 10(5)M-1 and 1.3 X 10(5)M-1 for P and Q respectively. The significance of these two parameters in relation to current theories of allostery is also discussed.
摘要
通过平衡透析和沉降研究，对催产素与牛神经垂体素II在pH 5.8的0.1M磷酸钠溶液中的相互作用进行了研究。结合曲线的S形归因于激素诱导或预先存在的异构化，催产素优先结合到一种异构状态。结果与形式为r = (2P[S]+2PQ[S]2)/(1+2P[S]+PQ[S]2)的结合方程一致，P和Q的值分别为0.7×10(5)M-1和1.3×10(5)M-1。还讨论了这两个参数与当前变构理论的关系。
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本文引用的文献
1
The interaction of ribonuclease with purine and pyrimidine phosphates. I. Binding of adenosine 5'-monophosphate to ribonuclease.核糖核酸酶与嘌呤及嘧啶磷酸盐的相互作用。I. 5'-磷酸腺苷与核糖核酸酶的结合
Biochim Biophys Acta. 1962 Mar 5;55:361-73. doi: 10.1016/0006-3002(62)90791-6.
2
ON THE NATURE OF ALLOSTERIC TRANSITIONS: A PLAUSIBLE MODEL.关于别构转变的本质：一个合理的模型。
J Mol Biol. 1965 May;12:88-118. doi: 10.1016/s0022-2836(65)80285-6.
3
[Oxytocin and vasopressin of sheep: reconstitution of an active hormonal complex].[绵羊的催产素和加压素：活性激素复合物的重组]
Biochim Biophys Acta. 1960 Feb 26;38:266-72. doi: 10.1016/0006-3002(60)91241-5.
4
Purification of oxytocin and vasopressin by way of a protein complex.通过蛋白质复合物纯化催产素和加压素。
J Biol Chem. 1958 Jul;233(1):116-20.
5
Fractionation of neurophysin by molecular-sieve and ion-exchange chromatography.通过分子筛和离子交换色谱法对神经垂体激素运载蛋白进行分级分离。
Biochem J. 1967 Jul;104(1):122-7. doi: 10.1042/bj1040122.
6
Comparison of experimental binding data and theoretical models in proteins containing subunits.含亚基蛋白质中实验结合数据与理论模型的比较。
Biochemistry. 1966 Jan;5(1):365-85. doi: 10.1021/bi00865a047.
7
Dissociation of oxytocin and vasopressin from their carrier protein by chromatography on sephadex G-25.通过葡聚糖凝胶G - 25柱层析法使催产素和加压素与其载体蛋白分离。
Br J Pharmacol Chemother. 1966 Feb;26(2):502-10. doi: 10.1111/j.1476-5381.1966.tb01931.x.
8
The isolation of the native hormone-binding proteins from bovine pituitary posterior lobes. Crystallization of neurophysin-I and-II as complexes with [8-arginine]-vasopressin.从牛垂体后叶中分离天然激素结合蛋白。神经垂体素-I和-II与[8-精氨酸]-加压素形成复合物的结晶。
Biochem J. 1968 Jan;106(2):557-64. doi: 10.1042/bj1060557.
9
The binding of oxytocin and oxytocin analogues by purified bovine neurophysins.纯化牛神经垂体素对催产素及其类似物的结合作用。
Proc Natl Acad Sci U S A. 1966 Aug;56(2):640-6. doi: 10.1073/pnas.56.2.640.
10
Complete amino acid sequence of bovine neurophysin II.牛神经垂体素II的完整氨基酸序列。
Biochem Biophys Res Commun. 1971 Jul 16;44(2):293-8. doi: 10.1016/0006-291x(71)90598-5.
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