McLaughlin Patricia J
Department of Neuroscience and Anatomy, The Milton S. Hershey Medical Center, Pennsylvania State University, Hershey, Pa 17033-0850, USA.
Biol Neonate. 2002;82(3):207-16. doi: 10.1159/000063611.
The influence of endogenous opioid blockade by naltrexone during prenatal life on postnatal heart development was studied in rats. Pregnant Sprague-Dawley rats received daily injections of 50 mg/kg naltrexone (NTX) or saline throughout gestation; offspring were cross-fostered at birth to mothers not receiving NTX. In general, NTX-treated offspring weighed more than controls throughout preweaning life, whereas heart weights were often increased from age-matched controls up to 35 days. Biochemical analyses of nucleic acids and protein demonstrated that DNA and protein content were increased throughout development in NTX-treated animals relative to controls. Morphometric analyses revealed increases in total area of the heart and myocardial area in NTX-exposed rats relative to control levels. These data suggest that endogenous opioids function to regulate cardiac growth during the prenatal period, and that disruption of this process has long-term implication for cardiac biology.
在大鼠中研究了孕期内源性阿片类物质被纳曲酮阻断对出生后心脏发育的影响。怀孕的斯普拉格-道利大鼠在整个妊娠期每天注射50毫克/千克纳曲酮（NTX）或生理盐水；幼崽出生时被交叉寄养给未接受NTX的母亲。一般来说，接受NTX治疗的幼崽在整个断奶前的生命过程中体重比对照组重，而心脏重量相对于年龄匹配的对照组，在35天之前常常增加。核酸和蛋白质的生化分析表明，与对照组相比，接受NTX治疗的动物在整个发育过程中DNA和蛋白质含量增加。形态计量分析显示，与对照水平相比，暴露于NTX的大鼠心脏总面积和心肌面积增加。这些数据表明，内源性阿片类物质在孕期发挥调节心脏生长的作用，而这一过程的破坏对心脏生物学具有长期影响。
