de la Calle Guntiñas M B, Bordin G, Rodriguez A R
European Commission, Joint Research Centre, Institute for Reference Materials and Measurements (IRMM), Retieseweg, 2440, Geel, Belgium.
Anal Bioanal Chem. 2002 Oct;374(3):369-78. doi: 10.1007/s00216-002-1508-3. Epub 2002 Sep 13.
The use of liquid chromatography in the separation and determination of metal-binding proteins is reviewed. Advantages and drawbacks of different chromatographic techniques based on various principles: size exclusion, ion exchange (cationic and ionic), reversed phase and affinity, are presented and discussed. The topic "metal-binding proteins" is considered and presented from two different points of view. The first one regards metal speciation in biological samples (serum and blood). In metal speciation studies, the exact identity of the protein to which the metal is bound often remains unknown. The second point of view is that, frequently, the interest of analyzing metal-binding proteins is not related anymore to the metallic fraction of the protein, but to other chemical structures attached to the protein, such as carbohydrates, which indirectly determine how good the function of the protein is. In this review, special attention is paid to studies dealing with the glycosylation of transferrin, and with the glycated isoform of haemoglobin.
本文综述了液相色谱法在金属结合蛋白分离与测定中的应用。介绍并讨论了基于不同原理的各种色谱技术（尺寸排阻色谱、离子交换色谱（阳离子和离子色谱）、反相色谱和亲和色谱）的优缺点。“金属结合蛋白”这一主题从两个不同的角度进行了探讨和阐述。第一个角度涉及生物样品（血清和血液）中的金属形态。在金属形态研究中，与金属结合的蛋白质的确切身份往往仍不明确。第二个角度是，通常情况下，分析金属结合蛋白的意义不再与蛋白质的金属部分相关，而是与附着在蛋白质上的其他化学结构有关，例如碳水化合物，它们间接决定了蛋白质功能的优劣。在这篇综述中，特别关注了有关转铁蛋白糖基化以及血红蛋白糖化异构体的研究。
