Arnaud Isabelle L, Josserand Jacques, Rossier Joël S, Girault Hubert H
Laboratoire d'Electrochimie, Département de Chimie, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
Electrophoresis. 2002 Sep;23(19):3253-61. doi: 10.1002/1522-2683(200210)23:19<3253::AID-ELPS3253>3.0.CO;2-4.
The protonation of an aqueous solution of two ampholytes AH and BH next to a gel buffered by immobilized acid moieties IH has been studied by finite element simulation in an iterative scheme. A ten species model has been formulated, taking into account transient diffusion and equilibrium kinetics of the two amphoteric species AH and BH, of water and of the immobilized species IH. This model has been developed to illustrate the pH evolution between an ampholyte solution and an Immobiline gel, and to study the influence of the Immobiline concentration on protons and ampholyte distributions. It has been demonstrated that a minimum initial Immobiline concentration of 10(-2) M is necessary to maintain the pH in the gel in contact with a closed chamber, when the difference between the isoelectric points of AH and BH is 4 and when the initial concentration of the ampholytes in solution is in the micromolar range. This approach provides a first theoretical framework for the recently developed Off-Gel trade mark electrophoresis.
通过迭代方案中的有限元模拟，研究了在由固定化酸基团IH缓冲的凝胶旁，两种两性电解质AH和BH的水溶液的质子化情况。已构建了一个包含十种物质的模型，该模型考虑了两种两性物质AH和BH、水以及固定化物质IH的瞬态扩散和平衡动力学。开发此模型是为了说明两性电解质溶液与Immobiline凝胶之间的pH演变，并研究Immobiline浓度对质子和两性电解质分布的影响。结果表明，当AH和BH的等电点之差为4且溶液中两性电解质的初始浓度处于微摩尔范围时，为了维持与封闭腔室接触的凝胶中的pH值，Immobiline的最低初始浓度必须为10(-2) M。这种方法为最近开发的Off-Gel商标电泳提供了首个理论框架。
