Ornskov Eivor, Ullsten Sara, Söderberg Lennart, Markides Karin E, Folestad Staffan
Department of Analytical Chemistry, Uppsala University, Uppsala, Sweden.
Electrophoresis. 2002 Sep;23(19):3381-4. doi: 10.1002/1522-2683(200210)23:19<3381::AID-ELPS3381>3.0.CO;2-4.
A novel procedure for immobilization of liposomes inside fused-silica capillaries is demonstrated. First, the inner wall of the capillaries was coated with a positively charged polymer, composed of derivatized agarose. Subsequently, negatively charged liposomes were immobilized by electrostatic interaction on the polymer coating. The developed liposome coated capillaries were used as a nanoseparation tool for studying interactions between small drug compounds and liposomes. Part of this work was presented at the 15th International Symposium on Microscale Separations and Analysis, HPCE 2002, Stockholm, Sweden, April 2002.
展示了一种将脂质体固定在熔融石英毛细管内的新方法。首先，用由衍生化琼脂糖组成的带正电荷聚合物涂覆毛细管内壁。随后，通过静电相互作用将带负电荷的脂质体固定在聚合物涂层上。所开发的脂质体包被毛细管被用作纳米分离工具，用于研究小药物化合物与脂质体之间的相互作用。这项工作的一部分在2002年4月于瑞典斯德哥尔摩举行的第15届国际微尺度分离与分析研讨会（HPCE 2002）上发表。
