McCleary Barry V, McNally Marian, Rossiter Patricia
Megazyme International Ireland Ltd., Bray, County Wicklow, Ireland.
J AOAC Int. 2002 Sep-Oct;85(5):1103-11.
Interlaboratory performance statistics was determined for a method developed to measure the resistant starch (RS) content of selected plant food products and a range of commercial starch samples. Food materials examined contained RS (cooked kidney beans, green banana, and corn flakes) and commercial starches, most of which naturally contain, or were processed to yield, elevated RS levels. The method evaluated was optimized to yield RS values in agreement with those reported for in vivo studies. Thirty-seven laboratories tested 8 pairs of blind duplicate starch or plant material samples with RS values between 0.6 (regular maize starch) and 64% (fresh weight basis). For matrixes excluding regular maize starch, repeatability relative standard deviation (RSDr) values ranged from 1.97 to 4.2%, and reproducibility relative standard deviation (RSDR) values ranged from 4.58 to 10.9%. The range of applicability of the test is 2-64% RS. The method is not suitable for products with <1% RS (e.g., regular maize starch; 0.6% RS). For such products, RSDr and RSDR values are unacceptably high.
针对一种用于测定特定植物性食品和一系列商业淀粉样品中抗性淀粉（RS）含量的方法，确定了实验室间的性能统计数据。所检测的食品原料含有抗性淀粉（煮熟的芸豆、青香蕉和玉米片）以及商业淀粉，其中大多数天然含有或经过加工后产生较高水平的抗性淀粉。所评估的方法经过优化，以得出与体内研究报告值一致的抗性淀粉值。37个实验室对8对盲法重复淀粉或植物材料样品进行了测试，抗性淀粉值在0.6%（普通玉米淀粉）至64%（鲜重计）之间。对于不包括普通玉米淀粉的基质，重复性相对标准偏差（RSDr）值在1.97%至4.2%之间，再现性相对标准偏差（RSDR）值在4.58%至10.9%之间。该测试的适用范围是2%至64%的抗性淀粉。该方法不适用于抗性淀粉含量低于1%的产品（如普通玉米淀粉；抗性淀粉含量为0.6%）。对于此类产品，RSDr和RSDR值高得令人无法接受。
