人胎盘基因表达谱DNA微阵列的初步研究。
Preliminary study of DNA microarray of human placenta gene expression profile.
作者信息
Zhu Li-Na, Ma Wen-Li, Mao Xiang-Ming, Li Ling, Feng Chun-Qiong, Zheng Wen-Ling
机构信息
Institute of Molecular Biology and Key Laboratory for Biochip of PLA, First Military Medical University, Guangzhou 510515, China.
出版信息
Di Yi Jun Yi Da Xue Xue Bao. 2002 May;22(5):400-2.
OBJECTIVE
To prepare human placenta genechip for analyzing differential gene expressions.
METHODS
The target gene amplified from Hybrid Hunter cDNA library was dotted onto the slides by the arrayer (PixSys 5500). Total RNA from K562 cells treated with or without arsenic trioxide was extracted, the mRNA purified and cDNA synthesized. Fluorescent labeling of the samples was performed by restriction display PCR, followed by hybridization with the microarray that was subsequently washed and scanned, with the derived signals analyzed by computer.
RESULTS
A reliable and specific method for preparing and assessing gene expression profile microarray was established, with which a total of 45 differentially expressed gene fragments were isolated.
CONCLUSION
The genechips can be helpful in the analysis of differential gene expressions.
目的
制备用于分析差异基因表达的人胎盘基因芯片。
方法
从杂交捕获cDNA文库中扩增出的靶基因通过点样仪（PixSys 5500）点样于玻片上。提取经三氧化二砷处理或未处理的K562细胞的总RNA，纯化mRNA并合成cDNA。通过限制性显示PCR对样品进行荧光标记，随后与微阵列杂交，微阵列经洗涤和扫描后，由计算机分析所得信号。
结果
建立了一种可靠且特异的制备和评估基因表达谱微阵列的方法，利用该方法共分离出45个差异表达的基因片段。
结论
该基因芯片有助于差异基因表达的分析。
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