Brandtner Eva-Maria, Lechner Thomas, Loidl Peter, Lusser Alexandra
Institute of Molecular Biology, University of Innsbruck, Fritz-Pregl-Str 3, 6020, Innsbruck, Austria.
Cell Biol Int. 2002;26(9):783-9. doi: 10.1016/s1065-6995(02)90933-9.
The dynamic state of post-translational acetylation of eukaryotic histones is maintained by histone acetyltransferases (HATs) and histone deacetylases (HDACs). HATs and HDACs have been shown to be components of various regulatory protein complexes in the cell. Their enzymatic activities, intracellular localization and substrate specificities are regulated in a complex, cell cycle related manner. In the myxomycete Physarum polycephalum multiple HATs and HDACs can be distinguished in biochemical terms and they exhibit dynamic activity patterns depending on the cell cycle stage. Here we report on the cloning of the first P. polycephalum HDAC (PpHDAC1) related to the S. cerevisiae Rpd3 protein. The expression pattern of PpHDAC1 mRNA was analysed at different time points of the cell cycle and found to be largely constant. Treatment of macroplasmodia with the HDAC inhibitor trichostatin A at several cell cycle stages resulted in a significant delay in entry into mitosis of treated versus untreated plasmodia. No effect of TSA treatment could be observed on PpHDAC1 expression itself.
真核生物组蛋白翻译后乙酰化的动态状态由组蛋白乙酰转移酶（HATs）和组蛋白去乙酰化酶（HDACs）维持。HATs和HDACs已被证明是细胞中各种调节蛋白复合物的组成成分。它们的酶活性、细胞内定位和底物特异性以一种复杂的、与细胞周期相关的方式受到调节。在黏菌多头绒泡菌中，从生化角度可以区分出多种HATs和HDACs，并且它们表现出依赖于细胞周期阶段的动态活性模式。在此，我们报道了首个与酿酒酵母Rpd3蛋白相关的多头绒泡菌HDAC（PpHDAC1）的克隆。在细胞周期的不同时间点分析了PpHDAC1 mRNA的表达模式，发现其基本保持恒定。在几个细胞周期阶段用HDAC抑制剂曲古抑菌素A处理大变形体，导致处理过的与未处理的变形体相比进入有丝分裂明显延迟。未观察到TSA处理对PpHDAC1表达本身有影响。
