Lu Jing, Tong Xiao-Yong, Wang Xiao-Liang
Department of Pharmacology, Institute of Materia Medica, Peking Union Medical College and Chinese Academy of Medical Sciences, 1 Xiannongtan Street, Beijing 100050, China.
Neurosci Lett. 2002 Oct 25;332(1):21-4. doi: 10.1016/s0304-3940(02)00905-9.
To investigate the effect of chronic global cerebral ischemia on gene expression of Na(+)/Ca(2+) exchanger isoforms NCX1, NCX2 and NCX3 in rat brain. Chronic global cerebral ischemia was induced by bilateral common carotid artery ligation (BCAL) in rats for 1 week, 2 weeks and 4 weeks, respectively. Morris water maze was applied to demonstrate the credibility of BCAL models. After BCAL for 4 weeks, there was learning and memory deficiency that the latency and distance of BCAL group were longer than those of sham group from the second trial to tenth trial in hidden platform trials. Reverse transcription-polymerase chain reaction was used to assess the gene expression of Na(+)/Ca(2+) exchanger isoforms at mRNA level in cerebral cortex and hippocampus. For NCX1, its expression was decreased by 35%, 54% and 27% of rats with BCAL for 1 week, 2 weeks and 4 weeks, respectively; For NCX2, its expression was decreased by 41%, 29% and 12% of rats with BCAL for 1 week, 2 weeks and 4 weeks, respectively; For NCX3, its expression was decreased by 29%, 27% and 12% of rats with BCAL for 1 week, 2 weeks and 4 weeks, respectively. However, in hippocampus, the expressions of NCX1 and NCX3 did not change significantly in different BCAL groups. NCX2 was increased by 60% in BCAL for 1 week only, but did not change significantly in BCAL for 2 weeks or 4 weeks. The study indicated that brain ischemia regulated gene expression levels of Na(+)/Ca(2+) exchanger isoforms especially in cerebral cortex.
研究慢性全脑缺血对大鼠脑内钠/钙交换体亚型NCX1、NCX2和NCX3基因表达的影响。分别通过双侧颈总动脉结扎（BCAL）诱导大鼠慢性全脑缺血1周、2周和4周。采用Morris水迷宫来验证BCAL模型的可靠性。BCAL 4周后，出现学习和记忆缺陷，在隐蔽平台试验中，从第二次试验到第十次试验，BCAL组的潜伏期和路程均长于假手术组。采用逆转录-聚合酶链反应在mRNA水平评估大脑皮质和海马中钠/钙交换体亚型的基因表达。对于NCX1，BCAL 1周、2周和4周的大鼠其表达分别下降35%、54%和27%；对于NCX2，BCAL 1周、2周和4周的大鼠其表达分别下降41%、29%和12%；对于NCX3，BCAL 1周、2周和4周的大鼠其表达分别下降29%、27%和12%。然而，在海马中，不同BCAL组的NCX1和NCX3表达无明显变化。仅BCAL 1周时NCX2增加60%，但BCAL 2周或4周时无明显变化。该研究表明脑缺血可调节钠/钙交换体亚型的基因表达水平，尤其是在大脑皮质。
