Firon Arnaud, d'Enfert Christophe
Institut Pasteur, Unité Microbiologie et Environnement, CNRS URA 2172, 25, rue du Dr Roux, Paris, France.
Trends Microbiol. 2002 Oct;10(10):456-62. doi: 10.1016/s0966-842x(02)02442-3.
Opportunistic fungal pathogens are an important cause of fatal invasive diseases and one of the many threats facing immunocompromised patients. Because of the limitations of the antifungal therapies currently available such as their toxicity, their narrow spectrum and the emergence of resistant pathogens, there is a significant demand for a broader antifungal arsenal. The characterization of genes essential for fungal growth will be an important step in the identification and development of novel antifungal drugs. Original strategies and new technologies including in vivo or in vitro transposon mutagenesis and post-transcriptional gene silencing are being developed for genome-scale identification of essential genes in fungal species that are pathogenic to humans.
机会性真菌病原体是致命侵袭性疾病的重要病因，也是免疫功能低下患者面临的众多威胁之一。由于目前可用的抗真菌疗法存在局限性，如毒性、谱窄以及耐药病原体的出现，对更广泛的抗真菌武器库有很大需求。鉴定对真菌生长至关重要的基因将是鉴定和开发新型抗真菌药物的重要一步。目前正在开发包括体内或体外转座子诱变和转录后基因沉默在内的原始策略和新技术，用于在基因组规模上鉴定对人类致病的真菌物种中的必需基因。
