诱发性热休克蛋白70表达对豚鼠耳蜗听力功能的影响。

Influence of evoked HSP70 expression on hearing function of the cochlea in guinea pigs.

作者信息

Wang Xu-Wu, Wang Xi-Jun, Song Jiang-Shun, Chen He-Xin, Man Yu-Hong

机构信息

Department of Otolaryngology, 456 Hospital of PLA, Ji'nan 250031, China.

出版信息

Di Yi Jun Yi Da Xue Xue Bao. 2002 Oct;22(10):922-4.

PMID:12377621

Abstract

OBJECTIVE

To investigate the effect of evoked heat shock protein 70 (HSP70) expression on the hearing function of the cochlea in guinea pigs.

METHODS

Guinea pigs were divided into pre-exposure group (pre-treated with white noise exposure at 100 dB SPL for 45 min) and none pre-exposure group. Auditory brainstem response (ABR) thresholds were recorded at 12, 60 and 108 h after the animals in both groups were exposed to loud white noise (125 dB SPL, 90 min).

RESULTS

HSP70 expression was evoked by pre-treatment with white noise (100 dB SPL, 45 min). There was no significant difference of ABR thresholds between the 2 groups (P>0.05) at 12 h after exposure to loud noise, while ABR thresholds became lower in pre-exposure group than in none pre-exposure group (P<0.01) at both 60 and 108 h. In the pre-exposure group, ABR thresholds at 108 h were significantly lower than that measured at 60 h (P<0.05), but which was not the case in none pre-exposure group.

CONCLUSION

HSP70 expression induced by pre-exposure to noise protects the hearing function of the cochlea in guinea pigs.

摘要

目的

探讨诱发性热休克蛋白70（HSP70）表达对豚鼠耳蜗听觉功能的影响。

方法

将豚鼠分为预暴露组（用100 dB SPL白噪声暴露预处理45分钟）和非预暴露组。两组动物暴露于高强度白噪声（125 dB SPL，90分钟）后12、60和108小时记录听性脑干反应（ABR）阈值。

结果

白噪声（100 dB SPL，45分钟）预处理可诱使HSP70表达。暴露于高强度噪声后12小时，两组ABR阈值无显著差异（P>0.05），而在60和108小时时，预暴露组ABR阈值低于非预暴露组（P<0.01）。在预暴露组中，108小时时的ABR阈值显著低于60小时时（P<0.05），但在非预暴露组中并非如此。