Ni Yue-Qiu, Tang Hao, Fu Wen-Shuang
Department of Physiology, Shenyang Medical College, Shenyang 110034, China.
Sheng Li Xue Bao. 2005 Jun 25;57(3):328-32.
To examine the significance of heat shock protein 70 mRNA in ototoxicity resulted from gentamicin (GM), twenty healthy albino guinea pigs (200-250 g) of either sex with a positive Prier reflex were divided into two groups randomly. In GM group the animals received 100 mg/kg GM daily by intraperitoneal injection for 10 d. In saline control group the animals received 2.5 ml/kg saline daily by intraperitoneal injection for 10 d. Auditory brainstem response (ABR) thresholds were recorded in each animal before and 1 d after GM or saline administration. After the second ABR measurement, the expression of HSP70 mRNA in guinea pig cochlea was observed with in situ hybridization and image quantitative analysis system. The results showed that the threshold of ABR in the GM group was significantly higher than that of the saline control (P< 0.001). The expression of HSP70 mRNA was more intensive in stria vascularis, spiral ligament and spiral ganglion cells in the GM group than that of the saline control group. These results suggest that administration of gentamicin can induce the expression of HSP 70 mRNA in guinea pig cochlea, and that this effect may protect hearing function from ototoxicity.
为研究热休克蛋白70信使核糖核酸（HSP70 mRNA）在庆大霉素（GM）所致耳毒性中的意义，将20只具有阳性普里尔反射、体重200 - 250克的健康白化豚鼠（雌雄不限）随机分为两组。GM组动物每天腹腔注射100毫克/千克GM，共10天。生理盐水对照组动物每天腹腔注射2.5毫升/千克生理盐水，共10天。在GM或生理盐水给药前及给药后1天记录每只动物的听性脑干反应（ABR）阈值。第二次ABR测量后，用原位杂交和图像定量分析系统观察豚鼠耳蜗中HSP70 mRNA的表达。结果显示，GM组的ABR阈值显著高于生理盐水对照组（P < 0.001）。GM组血管纹、螺旋韧带和螺旋神经节细胞中HSP70 mRNA的表达比生理盐水对照组更强烈。这些结果表明，庆大霉素给药可诱导豚鼠耳蜗中HSP70 mRNA的表达，且这种作用可能保护听力功能免受耳毒性影响。
