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双层趋化作用:无证据表明光子刺激人类血液多形核白细胞进行定向运动。

Double-decker chemotaxis: no evidence for photonic stimulation of directed locomotion by human blood polymorphonuclear leukocytes.

作者信息

Kaplow Leonard S, Chevance Ad Anne de Boisfleury, Henkel Joel, Malawista Stephen E

机构信息

Bradford, Vermont.

出版信息

Cell Motil Cytoskeleton. 2002 Dec;53(4):289-92. doi: 10.1002/cm.10074.

DOI:10.1002/cm.10074
PMID:12378539
Abstract

The study was carried out under direct videomicroscopic control to ascertain whether electromagnetic forces (photons) can initiate directed cell motility of human polymorphonuclear neutrophils (PMN). Cell suspensions containing a mixture of randomly motile white blood cells and erythrocytes (red cells) were placed in a double-decked preparation created by a glass slide and two cover slips and sealed by paraffin. Erythrocytes in the upper or lower chamber were destroyed by a single burst from a narrow ruby laser beam. Directed locomotion of PMN toward the erythrocyte debris occurred exclusively in the chamber in which the erythrocytes had been destroyed. Only random PMN locomotion was observed in the adjacent chamber. The results indicate that in this experimental model, electromagnetic forces do not initiate directed locomotion.

摘要

该研究是在直接视频显微镜控制下进行的,以确定电磁力(光子)是否能引发人类多形核中性粒细胞(PMN)的定向细胞运动。含有随机运动的白细胞和红细胞混合物的细胞悬液被置于由载玻片和两个盖玻片制成的双层制剂中,并用石蜡密封。上层或下层腔室中的红细胞被窄红宝石激光束的单次脉冲破坏。PMN向红细胞碎片的定向运动仅发生在红细胞被破坏的腔室中。在相邻腔室中仅观察到PMN的随机运动。结果表明,在该实验模型中,电磁力不会引发定向运动。

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