Mangakis N, Von Mickwitz C U
Pathol Eur. 1975;10(2):105-14.
Electron microscopical investigations by means of the ruthenium red method on Huggin-tumour cells cultivated in vitro resulted in a different thickness of the glycocalyx depending on the fixation of the cells in monolayer or, after mechanical detachment, in suspension. Cells fixed in suspension display a thicker glycocalyx, which is supposed to be due to a contraction of the cells before fixation. Tissue extracts from rapidly growing tissues (mammary gland of pregnant cows, tumour tissue) cause a significant reduction of the glycocalyx in transformed hamster fibroblasts and Huggin-tumour cells after an 18-hour period of action. These findings are in accordance with previous findings on the glycolytic effect of tissue extracts on sections of cartilaginous tissue. It is discussed whether the action of the tissue extracts on the glycocalyx is due to its detachment or to an inhibition of its renewal.
通过钌红法对体外培养的哈金肿瘤细胞进行电子显微镜研究发现，根据细胞在单层培养时的固定方式，或在机械分离后悬浮培养时的固定方式，糖萼的厚度有所不同。悬浮固定的细胞显示出较厚的糖萼，这被认为是由于固定前细胞的收缩所致。快速生长组织（怀孕母牛的乳腺、肿瘤组织）的组织提取物在作用18小时后，会导致转化的仓鼠成纤维细胞和哈金肿瘤细胞中的糖萼显著减少。这些发现与之前关于组织提取物对软骨组织切片糖酵解作用的研究结果一致。文中讨论了组织提取物对糖萼的作用是由于其脱离还是对其更新的抑制。
