胸膜肺炎放线杆菌RTX毒素ApxI的N端结构域在小鼠中引发保护性免疫。
The N-terminal domain of RTX toxin ApxI of Actinobacillus pleuropneumoniae elicits protective immunity in mice.
作者信息
Seah J N, Frey J, Kwang J
机构信息
Laboratory of Animal Health Biotechnology, Temasek Life Sciences Laboratory, The National University of Singapore, Singapore 117604, Singapore.
出版信息
Infect Immun. 2002 Nov;70(11):6464-7. doi: 10.1128/IAI.70.11.6464-6467.2002.
Abstract
We expressed three Actinobacillus pleuropneumoniae ApxI deletion derivatives to map the domain that could induce protective immunity. Antiserum to ApxI N-terminal covered by residues 40 to 380 was found to neutralize ApxI hemolytic activity but not ApxIII cytotoxicity. When used as a subunit vaccine in mice, this recombinant N-terminal fragment elicited protection against lethal infection with heterologous A. pleuropneumoniae serovars.
摘要
我们表达了三种胸膜肺炎放线杆菌ApxI缺失衍生物，以确定可诱导保护性免疫的结构域。发现针对由40至380位残基覆盖的ApxI N末端的抗血清可中和ApxI溶血活性，但不能中和ApxIII细胞毒性。当用作小鼠亚单位疫苗时，这种重组N末端片段可诱导针对异源胸膜肺炎放线杆菌血清型致死感染的保护作用。
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本文引用的文献
1
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2
Apx toxins in Pasteurellaceae species from animals.动物源巴斯德氏菌科物种中的Apx毒素
Vet Microbiol. 2000 Jun 12;74(4):365-76. doi: 10.1016/s0378-1135(00)00204-2.
3
Characterization of apxIVA, a new RTX determinant of Actinobacillus pleuropneumoniae.胸膜肺炎放线杆菌新RTX决定簇apxIVA的特性分析
Microbiology (Reading). 1999 Aug;145 ( Pt 8):2105-2116. doi: 10.1099/13500872-145-8-2105.
4
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Vaccine. 1999 Feb 5;17(5):441-7. doi: 10.1016/s0264-410x(98)00216-3.
5
Endobronchial inoculation with Apx toxins of Actinobacillus pleuropneumoniae leads to pleuropneumonia in pigs.用胸膜肺炎放线杆菌的Apx毒素进行支气管内接种可导致猪患胸膜肺炎。
Infect Immun. 1997 Oct;65(10):4350-4. doi: 10.1128/iai.65.10.4350-4354.1997.
6
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Infect Immun. 1996 Nov;64(11):4415-23. doi: 10.1128/iai.64.11.4415-4423.1996.
7
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Infect Immun. 1993 May;61(5):1682-6. doi: 10.1128/iai.61.5.1682-1686.1993.
8
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Mol Gen Genet. 1993 Oct;241(1-2):89-96. doi: 10.1007/BF00280205.
9
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Infect Immun. 1994 Apr;62(4):1262-7. doi: 10.1128/iai.62.4.1262-1267.1994.
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J Clin Microbiol. 1994 Nov;32(11):2749-54. doi: 10.1128/jcm.32.11.2749-2754.1994.
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