Lee P C, Schmidt-Dannert C
Department of Biochemistry, Molecular Biology and Biophysics, University of Minnesota, 1479 Gortner Avenue, St. Paul 55108, USA.
Appl Microbiol Biotechnol. 2002 Oct;60(1-2):1-11. doi: 10.1007/s00253-002-1101-x. Epub 2002 Aug 24.
Carotenoids are important natural pigments produced by many microorganisms and plants. Traditionally, carotenoids have been used in the feed, food and nutraceutical industries. The recent discoveries of health-related beneficial properties attributed to carotenoids have spurred great interest in the production of structurally diverse carotenoids for pharmaceutical applications. The availability of a considerable number of microbial and plant carotenoid genes that can be functionally expressed in heterologous hosts has opened ways for the production of diverse carotenoid compounds in heterologous systems. In this review, we will describe the recent progress made in metabolic engineering of non-carotenogenic microorganisms for improved carotenoid productivity. In addition, we will discuss the application of combinatorial and evolutionary strategies to carotenoid pathway engineering to broaden the diversity of carotenoid structures synthesized in recombinant hosts.
类胡萝卜素是许多微生物和植物产生的重要天然色素。传统上,类胡萝卜素已用于饲料、食品和营养保健品行业。最近发现类胡萝卜素具有与健康相关的有益特性,这激发了人们对生产结构多样的类胡萝卜素用于制药应用的极大兴趣。大量可在异源宿主中功能性表达的微生物和植物类胡萝卜素基因的可得性,为在异源系统中生产多种类胡萝卜素化合物开辟了道路。在本综述中,我们将描述在非类胡萝卜素生成微生物的代谢工程方面为提高类胡萝卜素产量所取得的最新进展。此外,我们将讨论组合和进化策略在类胡萝卜素途径工程中的应用,以拓宽重组宿主中合成的类胡萝卜素结构的多样性。