牛生长激素中羧基末端二硫键的选择性还原与烷基化
Selective reduction and alkylation of the COOH-terminal disulfide bridge in bovine growth hormone.
作者信息
Gráf L, Li C H, Bewley T A
出版信息
Int J Pept Protein Res. 1975;7(6):467-73. doi: 10.1111/j.1399-3011.1975.tb02467.x.
Abstract
Conditions leading to the cleavage of both disulfide bridges in human growth hormone caused the reduction of only one disulfide bond in bovine growth hormone. Partially reduced and alkylated derivatives of bovine growth hormone were prepared and characterized. It was shown that the reduction and alkylation modified the COOH-terminal disulfide bond, however, this modification does not result in the dissociation of the dimeric form of bovine growth hormone or cause a significant loss of growth-promoting activity.
摘要
导致人生长激素中两个二硫键断裂的条件只会使牛生长激素中的一个二硫键还原。制备并表征了牛生长激素的部分还原和烷基化衍生物。结果表明，还原和烷基化修饰了羧基末端的二硫键，然而，这种修饰并不会导致牛生长激素二聚体形式的解离，也不会导致促生长活性的显著丧失。
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